Enzymes Internal Assessment

Today we spent half the period discussing your first official Internal Assessment and the remaining half of the class reviewing your test on Topics 3.1-3.5 and 7.1-7.4.

You will be investigating the decomposition of hydrogen peroxide by catalase.  

On Wednesday March 7th, you must submit a completed Design for this experiment.  Essentially this Design would be suitable for submission to the IB.  You will have Friday March 9th to play in class and tweak your design.  You will be graded on the Design that you submit on March 7th and this will be the last grade you have before midterms.

Your Design should have:

A Title

Research Question & Background Information

Null Hypothesis (Ho) and Alternative Hypothesis (Ha)

Table that lists the Independent & Dependent Variables, as well as Controls.

Materials (with uncertainties)

Photograph of experimental set up ** You do not need to include this at this time, however you do need to identify a space for it in your paper. ** You do not need to include a protocol diagram.

Method (listed in steps).

A data table

A couple of things to keep in mind: 

* Your lab design can not be identical to another student (even if they are in a different period).

* If you research information on this topic, you must cite it in your Design.  This includes information contained in the Background section.

* As you are designing the experiment be mindful of how you will collect the data, since you will actually be doing this experiment.

* Internal Assessments count for almost 25% of your overall IB grade.  Since unlike a test, you know exactly what your Internal Assessment will cover, you should take the extra time to make it perfect.

* Refer to the rubric you received for the Osmosis Design Lab for more details on the requirements.

Enzymes

Yesterday and today we started our work on Enzymes.  Today we finished 3.6 (core).  You all have the pink calendar for the next four weeks so that you can see where we are going and when you will have time to work on your internal assessment.

Today we used the simulation below to look at the effects of manipulating temperature, pH and substrate concentration on enzyme activity.

Tomorrow we will be talking about what enzymes you can use for the experiment as well as the equipment that is available.

Class on Friday

Ms. Kavalauskas is covering E period and Mr. Kirsch is covering H period tomorrow.  You are expected to review your syllabi and prepare for your test.  Make sure you know the command terms so that you can correctly answer the questions.

If you need help, I will be checking my email while I am away, so if you have questions, you can email me.  Be specific in your questions, please don't email and say "I don't get______,"  what specifically are you having trouble understanding.

Connection: Consequences of errors in DNA

Sickle Cell Anemia:

Replacement of A with T at the 17th  nucleotide in a beta chain of hemoglobin.

The mutation changes from GAG (glutamic acid) to GTG (valine).

People with sickle cell anemia are resistant to malaria (very helpful in areas with a high rate of the disease).

Helpful Review Links

Since the International Food Fair is Thursday night and E period had some pretty good arguments for postponing the test, your test is now on Monday (for E period) or Tuesday (for H period).

Here are some links to help you review:

DNA Replication - silly
DNA Replication - also has review quizzes 
Transcription and Translation - Basic : From DNA Dolan Learning Center
Transcription and Translation - Advanced : From DNA Dolan Learning Center

How DNA is packaged -  From DNA Dolan Learning Center
Transcribe and Translate a Gene Game 

Macromolecules: an overview, primarily text
Macromolecules glossary
Lipids animation 
Functional groups
Chemistry overview and functional groups
Properties of Water
Chemistry Tutorial

H Period Homework Help

H period homework:

Please complete questions 9, 10 and 11 in the packet.  Use the PowerPoint and the links in the post below.

For question #9:

Click the picture to link to an animation and explanation on how tRNA is activated.
You do not need to know the term aminoacyl-tRNA synthetase. 

For Question #10:

The structure of a ribosome.

For Question #11

Visit Hartmut "Hudel" Leucke's lab page by click on his picture of a polysome, for a great explanation of how these ribosomes work together.

Topic 7: Transcription & Translation

Today we reviewed the core information on transcription and translation, before moving on to the details of these processes in topic 7.  Below is the PowerPoint from Mr. T for these sections.

Protein Synthesis: 7.3 & 7.4 Transcription and Translation AHL (IB)

View more presentations from Stephen Taylor

See an electron micrograph of a polysome (and for an explanation) here.

A detailed explanation of the activating enzyme reaction that joins the amino acid to the tRNA

A detailed explanation of the structure of tRNA.  This is far more detail than you need to know, but can be helpful in understanding the structure.

E period homework: Complete questions 6, 7 and 8 in the packet.

3.5 Transcription & Translation

Today we started to learn about transcription and translation.  We started by looking at the  Learn.Genetics Firefly overview and then watched this clip of translation from the DNA Learning Center.  We spent the rest the class going through the core material for Transcription and Translation (see the PowerPoint below).

Protein Synthesis: 3.5 Transcription & Translation (SL)  .

View more presentations from Stephen Taylor

For homework you need to complete the packet.  Please let's not have another "Most of you didn't do your homework so you have pop quiz" quiz.

Wrapping up Replication

Today E period had a "Most of you Didn't Do Your Homework, so You have a Pop Quiz" Quiz.  We spent the remainder of the period clarifying any questions on DNA replication.

Tomorrow we will start transcription and translation.  Remember to write in your planners that on February 17th (Friday) you will have a test on the current syllabus packet.  Topics are listed in the sidebar.

DNA Replication

Today we started DNA replication.  We used this animation to help us learn the basics.  St. Olaf's explanation, here, is also very easy to understand.  For homework, due Wednesday:  complete all of the packet, except the back of the last page.

Watch the Mechanisms of DNA replication below.  Without the sound can you identify which is the leading strand and which is the lagging strand?

7.1 - DNA Structure

Today we went into more detail on the structure of DNA, specifically addressing, how does all of that DNA fit into such a small space?!

Click to watch a video on how histones are used to package DNA

Tomorrow we will finish 7.1 and work on a review of 7.1 and 3.3.  Homework for tonight (due tomorrow):  Complete the DBQ on page 58 in your book.   This will be the last grade before interims!  Tomorrow you will get back your tests on Topic 2.